The article presents the results of qualitative and quantitative content of triterpene saponins in Salvia Stepposa Schost. raw materials. To identify triterpene compounds in raw materials, water-alcohol and aqueous extracts were used, in which their presence was determined using generally accepted qualitative reactions, and their relationship to the triterpene nature was also established. Quantitative determination of saponins in raw materials was determined by measuring the optical density of the obtained complexes in reaction with concentrated sulfuric acid. The content of triterpene saponins in the studied raw materials was: in leaves - 1.96%, in flowers - 1.90% and in grass - 2.02%.
Salvia stepposa Schost, triterpene saponins, identification reactions, quantitative determination, spectrophotometr
1. Ershova Yu.A., Damdinova Yu.P., Svirina M.M. Saponiny - biologicheski aktivnye soedineniya geranievyh // Prirodnye soedineniya i zdorov'e cheloveka: sbornik nauchnyh statey Vserossiyskoy nauchno-prakticheskoy konferencii: FGBOU VO IGMU Minzdrava Rossii. Irkutsk. 2021. S. 73-75.
2. Tchoukoua A., Douanla M.H., Ariefta N.R., Yoshida J., Ito Y., Ngadjui B.T., Shiono Y. Triterpene saponins from the roots of Acacia senegal (L.) Willd. // Fitoterapia. 2021. Vol. 151. P. 104859. doi:https://doi.org/10.1016/j.fitote.2021.104859.
3. Boyko N.N., Zaycev A.I., Osolodchenko T.P. Skrining antimikrobnyh svoystv spirto-vodnyh vytyazhek iz nekotoryh vidov rastitel'nogo syr'ya, soderzhaschego saponiny // Farmaciya Kazahstana. 2015. № 1 (164). S. 26-31.
4. Georgieva A., Popov G., Shkondrov A., Toshkova R., Krasteva I., Kondeva-Burdina M., Manov V.J. Antiproliferative and antitumour activity of saponins from Astragalus glycyphyllos on myeloid Graffi tumour // Ethnopharmacol. 2021. Vol. 267. P. 113519. doi:https://doi.org/10.1016/j.jep.2020.113519.
5. Jiang Liu, Zhang Jing, Wang Feng, Zou Yuan-feng, Che Xing-fu n. Isolation and characterization of new minor triterpenoid saponins from the buds of Lonicera macranthoides // Carbohydr Res. 2013. Vol. 370. P. 76-81. doi:https://doi.org/10.1016/j.carres.2013.01.019.
6. Linlin Bi, Tian Xiangrong, Dou Fang, Hong Liangjian, Tang Haifeng, Wang Siwang New antioxidant and antiglycation active triterpenoid saponins from the root bark of Aralia taibaiensis // Fitoterapia. 2012. Vol. 83(1). P. 234-240. doi:https://doi.org/10.1016/j.fitote.2011.11.002.
7. Kim You Ah, You Ah Kim, Chang-Suk Kong, Lee Jung Im, Kim Hojun, Hee Yeon Park. Evalution of novel antioxidant triterpenoid saponins from the halophyte Salicornia herbacea // Bioorganic and Medicinal Chemistry Letters. 2012. Vol. 22. P. 4318-4322.
8. Wu C., Duan Y.H., Tang W., Li M.M., Wu X., Wang G.C., Ye W.C., Zhou G.X., Li Y.L. New ursane-type triterpenoid saponins from the stem bark of Schefflera heptaphylla // Fitoterapia. 2014. Vol. 92. P. 127-132. doi:https://doi.org/10.1016/j.fitote.2013.10.006.
9. Zhou Xian-Li, Li Shan-Bin, Yan Meng-Qi, Luo Qin, Wang Li-Sheng, Shen Ling-Li, Liao Mei-Lian, Lu Cai-Hua, Liu Xia-Yan, Liang Cheng-Qin. Bioactive dammarane triterpenoid saponins from the leaves of Cyclocarya paliurus // Phytochemistry. 2021. Vol. 183. P. 112618. doi:https://doi.org/10.1016/j.phytochem.2020.112618.
10. Zhang X., Zou L.H., He Y.L., Peng C., Guo L., Xiong L. Triterpenoid saponins from the buds of Lonicera similis. // Nat Prod Res. 2018. Vol. 32(19). P. 2282-2290. doi:https://doi.org/10.1080/14786419.2017.1408092.
11. Liu L., Li H., Hu K., Xu Q., Wen X., Cheng K., Chen C., Yuan H., Dai L., Sun H. Synthesis and anti-inflammatory activity of saponin derivatives of δ-oleanolic acid // Eur J Med Chem. 2021. Vol. 1 (209). P. 112932. doi:https://doi.org/10.1016/j.ejmech.2020.112932.
12. Sarikahya N.B., Nalbantsoy A., Top H., Gokturk R.S., Sumbul H., Kirmizigul S. Immunomodulatory, hemolytic and cytotoxic activity potentials of triterpenoid saponins from eight Cephalaria species // Phytomedicine. 2018. Vol. 1(38). P. 135-144. doi:https://doi.org/10.1016/j.phymed.2017.11.009.
13. Sharma P., Tyagi A., Bhansali P., Pareek S., Singh V., Ilyas A., Mishra R., Poddar N.K. Saponins: Extraction, bio-medicinal properties and way forward to anti-viral representatives // Food Chem Toxicol. 2021. Vol. 150. P. 112075. doi:https://doi.org/10.1016/j.fct.2021.112075.
14. Shynkovenko I.L., Ilyina T.V., Kovalyova A.M., Goryacha O.V., Golembiovska O.I., Koshovyi O.M. Saponins of the extracts of Galium aparine and Galium verum // News of Pharmacy. 2018. № 4 (96). S. 16-23.
15. Zhongying Fang, Li Jia, Yang Ran, Fang Lei, ZhangA Yongqing. Review: The Triterpenoid Saponins and Biological Activities of Lonicera Linn. // Molecules. 2020. Vol. 25(17). P. 3773. doi:https://doi.org/10.3390/molecules25173773.
16. Mironenko N.V., Selemenev V.F. Razrabotka sposobov identifikacii i kolichestvennogo opredeleniya saponinov Sapindus mukorossi // Epistemologicheskie osnovaniya sovremennogo obrazovaniya: aktual'nye voprosy prodvizheniya fundamental'nogo znaniya v uchebnyy process: materialy Mezhdunarodnoy nauchno-prakticheskoy konferencii Borisoglebskogo filiala FGBOU VO «VGU». Moskva. 2020. S. 377-382.
17. Moiseev Ya.P., Kurdyukov E.E., Mitishev A.V., Vodop'yanova O.A., Rodina O.P., Zhuchenko E.V. Spektrofotometricheskoe opredelenie summy saponinov v plodah derezy kitayskoy Lycium chinense mill // Himiya rastitel'nogo syr'ya. 2021. № 2. C. 123-128.
18. El' M.H., Kauhova I.E., Sorokin V.V., Minina S.A. Razrabotka metodiki kolichestvennogo opredeleniya saponinov v trave gryzhnika gologo Herniaria glabra l. // Nauchnye vedomosti Belgorodskogo gosudarstvennogo universiteta. Seriya: Medicina. Farmaciya. 2014. № 24 (195). S. 235-238.
19. Kotelnaya Ya.I., Alekhina E.A., Efremov A.N., Bolotova Ya.V., Guselnikova M.V., Nikolaenko S.A., Toma C. B. Notes on the saponins in the plants of the family Hydrocharitaceae // Botanica Pacifica: a Journal of Plant Science and Conservation. 2019. T. 8. № 1. - S. 57-61.
20. Yusifova D.Yu., Movsumov I.S. Flavonoidy i triterpenovye saponiny Scabiosa hyrcanica stev., proizrastayuschey v Azerbaydzhane // Himiya rastitel'nogo syr'ya. 2015. № 2. S. 261-264.
21. Popova T.S., Tereshina N.S. Triterpenovye saponiny v list'yah i pochkah chernoy smorodiny // Farmaciya. 2015. № 8. S. 3-5.
